High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma

An isocratic high-performance liquid chromatography (HPLC) method was developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3 ml of plasma and involves extraction with dichloromethane. The HPLC separation was carried out on Symmetry Shield RP18, a mobile phase of methanol–water–acetic acid (65:35:0.2) and fluorescence detection at λex=410 nm and λem=510 nm. The retention time of AE was 11.7 min. The assay was linear from 10 to 1000 ng/ml (r2≥0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3–105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5 ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.