Author/Authors :
Ksyci?ska، نويسنده , , Hanna and Sobik، نويسنده , , Barbara and Popio?kiewicz، نويسنده , , Joanna and Polkowski، نويسنده , , Krzysztof and Krzeczy?ski، نويسنده , , Piotr and Ramza، نويسنده , , Jan and Pucko، نويسنده , , Wies?aw and Grynkiewicz، نويسنده , , Grzegorz، نويسنده ,
Abstract :
Methods for determination of genistein and its four new analogues in culture media have been developed to support studies on their potential anticancer activities. The investigated compounds were extracted from the media using liquid–liquid extraction with appropriate solvent. After evaporation of organic solvents each of the dry extracts was reconstituted in appropriate mobile phase. Reversed-phase HPLC was applied to quantitative determining of tested compounds. The methods are specific, sensitive and technically simple. They were used to evaluate concentration level of investigated compounds in experiments with human promyelocytic leukemia cells (HL-60 cell line).
