Title of article :
Optimization of bovine serum albumin sorption and recovery by hydrogels
Author/Authors :
Gündüz، نويسنده , , Ufuk and Tolga، نويسنده , , Asl?، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
4
From page :
13
To page :
16
Abstract :
Aqueous two-phase systems are composed of aqueous solutions of either two water-soluble polymers, usually polyethylene glycol (PEG) and dextran (Dx), or a polymer and a salt, usually PEG and phosphate or sulfate. Partitioning of proteins in such systems provides a powerful method for separating and purifying mixtures of biomolecules by extraction. If one of the phase forming polymers is a crosslinked gel, then the solution-controlled gel sorption may be considered as a modification of aqueous two-phase extraction. Since PEG/dextran systems are widely used in aqueous two-phase extraction and dextran gels (Sephadex) are common chromatographic media, we choose a PEG/dextran gel system as a model system in this study. The partitioning behavior of pure bovine serum albumin (BSA) in PEG/dextran gel systems is investigated to see the effects of variations in PEG and NaCl concentrations on the partition coefficient K. By making use of the Box–Wilson experimental design, K is shown to be maximized at 9.8 (%, w/w) PEG and 0.2 M NaCl concentrations, respectively, as 182.
Keywords :
Box–Wilson experimental design , polyethylene glycol , sephadex , Aqueous two-phase system , Bovine serum albumin
Journal title :
Journal of Chromatography B
Serial Year :
2004
Journal title :
Journal of Chromatography B
Record number :
1456815
Link To Document :
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