Title of article
Determination of intracellular concentrations of the TRPM2 agonist ADP-ribose by reversed-phase HPLC
Author/Authors
Gasser، نويسنده , , Andreas H. Guse، نويسنده , , Andreas H.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
7
From page
181
To page
187
Abstract
Since the NAD metabolite ADP-ribose (ADPR) has recently gained attention as a putative messenger, a method was established for the quantification of intracellular ADPR by reversed-phase HPLC. Cellular nucleotides were extracted with trichloroacetic acid, and crude cell extracts purified by solid phase extraction using a strong anion exchange matrix. After optimization of the extraction procedure, cellular ADPR levels were determined using two different reversed-phase columns (C18 versus C12), operated in ion pair mode. Intracellular ADPR concentrations in human Jurkat T-lymphocytes and murine BW5147 thymocytes were determined to be 44 ± 11 μM and 73 ± 11 μM, respectively.
Keywords
ADP-ribose , TRPM2 , KCa channels , HPLC , Calcium signaling , Solid phase extraction
Journal title
Journal of Chromatography B
Serial Year
2005
Journal title
Journal of Chromatography B
Record number
1459080
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