Inclusion of the standard deviation of data in spectral mapping. A case study

Colour pigments of paprika powders of different origin were separated and quantitatively determined by reversed-phase high performance liquid chromatography. The standard deviations were included in the matrices of retention data and the matrices were evaluated by spectral mapping technique followed by two-dimensional nonlinear mapping. The resuls indicated that the distribution of paprika samples on the two-dimensional selectivity map is a better indicator of the origin of the samples, compared to what the potency values do, and the inclusion of the standard deviation of raw data may be employed as a useful tool to enhance the reliability of the authenticity test of paprika samples.