Title of article
Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering
Author/Authors
Zhou، نويسنده , , Weibin and Bi، نويسنده , , Jingxiu and Janson، نويسنده , , Jan-Christer and Li، نويسنده , , Yan and Huang، نويسنده , , Yongdong and Zhang، نويسنده , , Yan and Su، نويسنده , , Zhiguo، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
7
From page
71
To page
77
Abstract
The molecular weight and size of recombinant Hepatitis B surface antigen (HBsAg) derived from Chinese hamster ovary (CHO) and the Hansenula polymorph have been characterized by high-performance size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALLS). The average molecular weight of CHO-derived HBsAg particle (CHO-rHBsAg) (4921 kDa) was higher than that of H. polymorpha yeast strain (Hans-rHBsAg) (3010 kDa). The size of CHO-rHBsAg (22.1 nm) is nearly the same as that of native HBsAg compared to 18.1 nm for Hans-rHBsAg. The average monomer numbers were found to be 155 for CHO-rHBsAg and 86 for Hans-rHBsAg, respectively. The data obtained support the assumption that the higher immunogenicity of CHO-derived HBsAg is related to its more favorable macromolecular assembly structure.
Keywords
Hepatitis B Surface Antigen (HBsAg) , Distribution , Molecular size , HPSEC-MALLS , molecular weight
Journal title
Journal of Chromatography B
Serial Year
2006
Journal title
Journal of Chromatography B
Record number
1463239
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