Author/Authors :
Zendelovska، نويسنده , , Dragica and Simeska، نويسنده , , Suzana and Sibinovska، نويسنده , , Olgica and Kostova، نويسنده , , Elena and Milo?evska، نويسنده , , Kalina and Jakovski، نويسنده , , Krume and Jovanovska، نويسنده , , Emilija and Kikerkov، نويسنده , , Igor and Troja?anec، نويسنده , , Jasmina and Zafirov، نويسنده , , Dim?e، نويسنده ,
Abstract :
Nifedipine, a dihydropyridine calcium channel antagonist, is widely used in the treatment of hypertension and other cardiovascular disorders. A selective, sensitive and accurate high-performance liquid chromatographic method has been developed, validated and applied for determination of nifedipine in human plasma samples. A series of studies were conducted in order to investigate the effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers, and to develop a convenient and easy-to-use method for quantitative analysis of nifedipine. The method involves solid-phase extraction on C18 cartridges. The chromatographic separation was accomplished on a Lichrocart Lichrospher 60 RP selectB column with a mobile phase composed of 0.020 mol/L KH2PO4 (pH 4.8) and acetonitrile (42:58, v/v). UV detection was set at 240 nm. The calibration curve was linear in the concentration range of 5.0–200.0 ng/mL for nifedipine in plasma and the limit of quantification was 5.0 ng/mL.
