Simultaneous determination of 5-hydroxyindoleacetic acid and 5-hydroxytryptamine in urine samples from patients with acute appendicitis by liquid chromatography using poly(bromophenol blue) film modified electrode

The fabrication and application of a novel electrochemical detection (ED) system with a poly(bromophenol blue) (PBPB) film chemically modified electrode (CME) for high performance liquid chromatography (HPLC) were described. The electrochemical behaviors of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) at this CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that the PBPB CME efficiently exhibited electrocatalytic effect on the current responses of 5-HT and 5-HIAA with relatively high sensitivity, stability and long life of activity. In HPLC–ED, the two analytes had good and stable current responses at the CME and their linear ranges were over four orders of magnitude (R ≥ 0.9992) with the detection limits being 0.25 nmol L−1 for 5-HT and 0.50 nmol L−1 for 5-HIAA. The application of this method for the determination of 5-HT and 5-HIAA in urine samples from patients with acute appendicitis (AA) was satisfactory.