FTMS and TOF/TOF mass spectrometry in concert: Identifying peptides with high reliability using matrix prespotted MALDI target plates

In this paper we describe a combination of the mass spectrometric techniques MALDI–TOF/TOF and MALDI–FTMS to identify proteins in complex samples using prespotted MALDI target plates. By this procedure accurate FTMS mass measurements and TOF/TOF data are obtained from the same spot. We have found that this combination of techniques leads to more reliable identification of peptides.