Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography–mass spectrometry for the measurement of progesterone in human serum

Miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) and gas chromatography–mass spectrometry (GC–MS) for the determination of progesterone in human serum sample is described. The detection limit and the quantification limit of progesterone in human serum sample are 0.5 and 2 ng mL−1 (ppb), respectively. The calibration curve for progesterone is linear with a correlation coefficient of >0.999 in the range of 2–500 ng mL−1. The average recoveries of progesterone in human serum samples spiked with 5, 50 and 200 ng mL−1 progesterone are 97.4% (R.S.D.: 9.3%), 100.3% (R.S.D.: 4.7%) and 99.8% (R.S.D.: 3.4%), respectively. This simple, accurate, sensitive and selective analytical method can be applicable for the determination of progesterone in human serum samples.