Title of article
A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat
Author/Authors
van den Broeck، نويسنده , , Hetty C. and America، نويسنده , , Antoine H.P. and Smulders، نويسنده , , Marinus J.M. and Bosch، نويسنده , , Dirk and Hamer، نويسنده , , Rob J. and Gilissen، نويسنده , , Ludovicus J.W.J. and van der Meer، نويسنده , , Ingrid M.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2009
Pages
8
From page
975
To page
982
Abstract
The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN® Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
Keywords
R5 , Celiac disease , Monoclonal antibodies , Gliadins , Glutenins , Immunoblotting , Gluten protein extraction , Wheat
Journal title
Journal of Chromatography B
Serial Year
2009
Journal title
Journal of Chromatography B
Record number
1467036
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