Title of article
An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE)
Author/Authors
Rafamantanana، نويسنده , , M.H. and Rozet، نويسنده , , E. and Raoelison، نويسنده , , G.E. and Cheuk، نويسنده , , K. and Ratsimamanga، نويسنده , , S.U. and Hubert، نويسنده , , Ph. and Quetin-Leclercq، نويسنده , , J.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2009
Pages
7
From page
2396
To page
2402
Abstract
The simultaneous quantification of madecassoside, asiaticoside, madecassic acid and asiatic acid in Centella asiatica by HPLC-UV is proposed. Asiaticoside was used as reference for the quantification of heterosides and asiatic acid for aglycones. The evaluation of the extraction efficiency of the four molecules led to use Soxhlet extraction for 8 h. The method was validated and was found to be accurate in the concentration range of 1.0–3.0 mg/ml for asiaticoside and 0.5–2.0 mg/ml for asiatic acid with CV <3% for all investigated compounds. LOD and LOQ were, respectively, 0.0113 and 1.0 mg/ml for asiaticoside and 0.0023 and 0.5 mg/ml for asiatic acid. This method was shown to be convenient for routine analysis of samples of C. asiatica.
Keywords
Centella asiatica , Asiaticoside , Madecassic acid , Asiatic acid , Method validation , Quantification , Madecassoside
Journal title
Journal of Chromatography B
Serial Year
2009
Journal title
Journal of Chromatography B
Record number
1467449
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