Author/Authors :
Nicholas P. and Petrikovics، نويسنده , , Ilona and Yu، نويسنده , , Jorn C.C. and Thompson، نويسنده , , David E. and Jayanna، نويسنده , , Prashanth and Logue، نويسنده , , Brian A. and Nasr، نويسنده , , Jessica and Bhandari، نويسنده , , Raj K. and Baskin، نويسنده , , Steven I. and Rockwood، نويسنده , , Gary، نويسنده ,
Abstract :
2-Aminothiazoline-4-carboxylic acid (ATCA) was intravenously injected to rats in order to investigate its plasma distribution. ATCA was extracted from plasma samples by solid phase extraction (SPE) and molecularly imprinted polymer stir bar sorption extraction (MIP-SBSE). Detection and quantification of ATCA were achieved by using liquid chromatography–tandem mass spectrometry (LC–MS/MS). It was found that the intravenously injected ATCA concentration quickly decreased to half within 2.5 h in the rat system. However, after 2.5 h, the concentration of ATCA in plasma stayed constant at least 5 folds above the endogenous ATCA level for more then 48 h. This finding can be used for evaluating ATCAʹs diagnostic and forensic value as a biomarker for cyanide exposure.
Keywords :
Diagnostic biomarker , Forensic biomarker , Cyanide exposure , 2-Aminothiazoline-4-carboxylic acid (ATCA) , LC–MS/MS
