Simultaneous determination of selected chemotherapeutics in human whole blood by molecularly imprinted polymers coated solid phase microextraction fibers and liquid chromatography–tandem mass spectrometry

Development and validation of novel, general liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the simultaneous determination of amoxicillin (AMOX), cefatoxime (CEF), ciprofloxacin (CIP), daptomycin (DAPTO), fluconazole (FLU), gentamicin (GEN), clindamycin (KLI), linezolid (LIN), metronidazole (MET), moxifloxacin (MOXI) in human whole blood are described. Samples were prepared on solid phase microextraction way with the use of polymeric sorption coatings with molecular imprints and analyzed using a gradient separation over an ACE C18-column (4.6 mm × 150 mm, 3 μm) with isocratic elution. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile–water (0.1% formic acid or 5 mM ammonium acetate) at a flow 0.4 ml/min. The chromatographic run time was kept less than 9 min. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 7.3%, while the accuracy was within ±8.4%. The mean recovery of all the analytes ranged from 65.0 to 83.0%. This method was successfully applied to clinical samples from patients with clinically diagnosed bacterial infections process.