Title of article
Evidence for post-mortem m-calpain autolysis in porcine muscle
Author/Authors
Pomponio، نويسنده , , Luigi and Lametsch، نويسنده , , René and Karlsson، نويسنده , , Anders H. and Costa، نويسنده , , Leonardo Nanni and Grossi، نويسنده , , Alberto and Ertbjerg، نويسنده , , Per، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
4
From page
761
To page
764
Abstract
The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.
Keywords
Calpains , Zymography , autolysis , pork , Post-mortem changes
Journal title
Meat Science
Serial Year
2008
Journal title
Meat Science
Record number
1488572
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