Author/Authors :
Hua، نويسنده , , G.H. and Chen، نويسنده , , S.L. and Yu، نويسنده , , J.N. and Cai، نويسنده , , K.L. and Wu، نويسنده , , C.J and Li، نويسنده , , Q.L. and Zhang، نويسنده , , C.Y. and Liang، نويسنده , , A.X. and Han، نويسنده , , L. S. Geng ، نويسنده , , L.Y. and Shen، نويسنده , , Z. and Xu، نويسنده , , D.Q. and Yang، نويسنده , , L.G.، نويسنده ,
Abstract :
In the present study, the polymorphism of growth hormone (GH) gene was analyzed as a genetic marker candidate for growth traits in Boer goat bucks. Two single nucleotide polymorphisms (SNPs) – A781G (Ser/Gly35) and A1575G (Leu147), were identified by GH gene sequencing and PCR–RFLP (polymerase chain reaction–restriction fragment length polymorphism) analysis. AA genotype resulted in a significant decrease in birth chest girth (P = 0.03) and weaning weight (P = 0.014) comparing to AB genotype, while CC genotype contributed to weaning height (P = 0.04) greater than CD genotype. When in combination, AACD genotype was undesired for lower scores in a series of growth traits including body weight, length, height, and chest girth at birth and weaning, as well as the pre-weaning daily gain and body weight at age of 11 months. These results indicate that new molecular markers associated with caprine growth traits can be used in MAS (marker-assisted selection) in Boer goat bucks.
Keywords :
Growth hormone , Boer goat , Growth , Polymorphism , PCR–RFLP
