Title of article :
Effect of ±5?C difference from physiologic temperature on surface area, volume, and surface-to-volume ratio of transformed cells
Author/Authors :
Rezaei-Tavirani، M نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Rezaei-Tavirani, M , Kalantari، S نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Kalantari, S , Hessami Tackallou، S نويسنده Department of Biology, Islamic Azad University, Garmsar Branch, Garmsar, Iran Hessami Tackallou, S , Rastmanesh، R نويسنده Human Nutrition Department, Faculty of Nutrition, Shahid Beheshti University of Medical Sciences, Tehran, Iran Rastmanesh, R
Abstract :
Information on the relationship between culture temperature and changes in the cellʹs area, volume and area to
volume (S/V) ratio is lacking. Cancerous epithelial-like cell lines SW480, SW742 and T-47D were cultured at 32,
37 and 42°C for 48hr. Digital images of cells were analyzed using software Imaged. Observed surface area,
volume changes, and surface area to volume (S/V) ratio were significantly different at 32, 37 and 42°C in the three
cell-lines tested (P < 0.05). S/V ratios for all three cell lines at 37°C were significantly greater than that of at 42°C
(P=0.0001).
