Title of article
Analysis of oligomannuronic acids and oligoguluronic acids by high-performance anion-exchange chromatography and electrospray ionization mass spectrometry
Author/Authors
Liu، نويسنده , , Yan and Jiang، نويسنده , , Xiao-Lu and Cui، نويسنده , , He and Guan، نويسنده , , Hua-Shi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
7
From page
105
To page
111
Abstract
Oligomannuronic acids and oligoguluronic acids were prepared by enzymatic hydrolysis of alginate with alginate lyases. The oligosaccharides generated up to degree of polymerization 16 were characterized by high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) and electrospray ionization mass spectrometry (ESI-MS). Acetate buffer linear gradients were used as mobile phases for separations of oligosaccharides. ESI-MS and HPAEC–PAD are very effective for the analysis and characterization of anionic oligosaccharides.
Keywords
Polysaccharides , Oligomannuronic acids , Oligoguluronic acids
Journal title
Journal of Chromatography A
Serial Year
2000
Journal title
Journal of Chromatography A
Record number
1501777
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