Author/Authors :
Deshmukh، نويسنده , , Ranjit R and Warner، نويسنده , , Timothy N and Hutchison، نويسنده , , Fred and Murphy، نويسنده , , Michael and Leitch II، نويسنده , , William E and De Leon، نويسنده , , Patricia and Srivatsa، نويسنده , , Susan P.C. Cole، نويسنده , , Douglas L and Sanghvi، نويسنده , , Yogesh S، نويسنده ,
Abstract :
Very high flux ion-exchange membranes were utilized for a novel purification of antisense oligonucleotides (20-mer). Strong anion-exchange membranes were produced by attaching polymeric ligands onto a microporous cellulosic matrix. The oligonucleotides purified were therapeutic single-stranded phosphorothioates deoxyribonucleotides. Although small-scale membrane devices (15 cm2) had similar resolution to traditional chromatographic columns; their throughputs were superior. Greater than a 1300-fold scale-up produced very similar purity and yields of the phosphorothionate product. Scale-up experiments were conducted with a 2 m2 surface area membrane module. These modules were easily capable of very high throughputs of 0.5 to 2 l/min. High purity and yields were achieved by both step and linear gradient elution.
