Comparison of phenyl-type columns in the development of a fast liquid chromatographic system for eighteen opiates commonly found in forensic toxicology

We report a precise and reliable method for the detection of 18 of the most commonly found opiates on the Belgian legal and illicit market, by ion-exchange, reversed-phase high-performance liquid chromatography, using a conventional phenyl-type analytical column (150×4.6 mm I.D., particle size 5 μm) and diode-array detection. We also describe a performance (efficiency and sensitivity) comparison of this column to a recently developed “high-speed” column (53×7.0 mm I.D., particle size 3 μm) packed with the same stationary phase, and used under slightly adjusted flow and gradient conditions. The final method, using the “high-speed” column, showed a significant reduction (55%) in analysis time without loss of resolution and sensitivity.