Author/Authors :
Shangguan، نويسنده , , Dihua and Han، نويسنده , , Huiwan and Zhao، نويسنده , , Rui and Zhao، نويسنده , , Yingxin and Xiong، نويسنده , , Shaoxiang and Liu، نويسنده , , Guoquan، نويسنده ,
Abstract :
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C24–C25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized products were separated by RP-HPLC with gradient elution. The detection limits of ginsenosides Rg1 and Rb1 were 2.0 ng (about 2.5 pmol) and 1.0 ng (about 0.9 pmol), respectively. This method can be used for all ginsenosides having the C24–C25 double bond.
