Title of article
Capillary isoelectric focusing with UV-induced fluorescence detection
Author/Authors
Hork?، نويسنده , , Marie and Willimann، نويسنده , , Thomas and Blum، نويسنده , , Monika and Nording، نويسنده , , Pierre and Friedl، نويسنده , , Zden?k and ?lais، نويسنده , , Karel، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
7
From page
65
To page
71
Abstract
Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV photometric and UV excited fluorometric detection. The experimental set-up of capillary IEF with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented.
Keywords
Proteins
Journal title
Journal of Chromatography A
Serial Year
2001
Journal title
Journal of Chromatography A
Record number
1507808
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