Chromatographic separation of tocopherols

α-, β-, γ-, and δ-Tocopherols were separated by reversed-phase high-performance thin-layer chromatography (C18RP-HPTLC), normal-phase high-performance liquid chromatography (NP-HPLC), reversed-phase high-performance liquid chromatography (C18RP-HPLC), and gas chromatography (GC). The selected topological indices based on connectivity (M, 1χν), on distance matrix (W, °B, MTI) and on information theory (IAC, ĪAC) were calculated for these tocopherols. The observed chromatographic separations of investigated tocopherols were compared. This comparison indicated that the C18RP-HPTLC, NP-HPLC, and GC are the best techniques for the separation of these tocopherols. Topological index °B was the most significant. We obtained definite dependence between the numerical values of topological index °B and the chromatographic separation of the investigated tocopherols.