Analysis of purines and pyrimidines by mixed partition–adsorption normal-phase high-performance liquid chromatography

This paper summarizes the results in the development of mixed partition–adsorption (MPA) normal-phase high-performance liquid chromatography published in the last 10 years. The MPA normal-phase systems are an alternative approach not only to the adsorption normal-phase mode but also to the most widely used reversed-phase mode in the separation area of purine and pyrimidine derivatives. It is shown that the MPA systems are applicable in analytical practice.