Solid-phase microextraction–gas chromatographic–mass spectrometric method for the determination of the fungicides cyprodinil and fludioxonil in white wines

A solid-phase microextraction (SPME) followed by a gas chromatographic–mass spectrometric (GC–MS) determination has been developed and validated for the determination of cyprodinil and fludioxonil in white wine samples. Extraction parameters such as the selection of SPME coating, the effect of the temperature, the effect of the headspace volume and the salt addition were studied and optimized, together with GC–MS analytical conditions. The divinylbenzene–Carboxen–polydimethylsiloxane (DVB–CAR–PDMS) fiber was the most appropriate for the determination of the two pesticides in wine. The quality parameters of the proposed method demonstrated a good precision (RSD about 5%), with detection limits of 0.1 and 0.2 μg/l for cyprodinil and fludioxonil, respectively. Fifteen commercial white wine samples produced in Rı́as Baixas area in Galicia (N.W. Spain) were analyzed with the SPME–GC–MS procedure. Some of the commercial wines (75%) presented the two pesticides in concentrations ranging from 0.9 to 28.6 μg/l. In conclusion, SPME–GC–MS has a great potential for fungicide determination in wines.