Performance and scale-up of adsorptive membrane chromatography

Separation efficiency and scalability of Pall Corporation’s new Mustang stacked membrane chromatographic devices were investigated, using both the 10-ml and 1-l models and comparing the responses of tracer pulses obtained for conventional and reverse-flow operation. Tracers included AMP, lysozyme, and thyroglobulin, which vary in relative molecular mass from less than 1000 up to 650 000. Both devices showed marked insensitivity to tracer size and flow-rate and gave sharper peaks than would have been expected from conventional 15-μm bead packings. However, reverse-flow peaks were always significantly sharper than those for conventional operation, and the differences were ascribed primarily to non-uniform header residence times. Numerical simulations of the macroscopic flow confirmed that this was indeed the case. This problem was much less pronounced for the 1-l device so scale-up is conservative.