Ultra-low-temperature non-aqueous capillary electrophoretic separation–77 K fluorescence spectroscopic detection for the on-line identification of photo-converted analytes of trans-resveratrol

We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE–FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼−70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE–FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE–FS are also briefly addressed.