Title of article :
Microchip electrophoresis of bacteria using lipid-based liquid crystalline nanoparticles
Author/Authors :
Wang، نويسنده , , Zhi-Fang and Cheng، نويسنده , , Shuang and Ge، نويسنده , , Shu-Li and Zhu، نويسنده , , Jin-Kun and Wang، نويسنده , , Huan and Chen، نويسنده , , Qiming J. Wang، نويسنده , , Qing-Jiang and He، نويسنده , , Pingang and Fang، نويسنده , , Yu-Zhi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
8
From page :
8281
To page :
8288
Abstract :
The aggregation and adhesion of bacterial cells is a serious disadvantage for electrophoretic separations of bacteria. In this study, lipid-based liquid crystalline nanoparticles were used as a pseudostationary phase to minimise the bacterial aggregation and adsorption to the inner walls of microchannels. Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus and Lactobacillus rhamnosus were selected as analytes and were separated by microchip electrophoresis (MCE) with laser-induced fluorescence (LIF) detection using 4.5 mM tris(hydroxymethyl) aminomethane (TRIS)–4.5 mM boric acid–0.1 mM ethylenediaminetetraacetate (EDTA) (TBE) containing poly(ethylene oxide) (PEO) and lipid-based nanoparticles as the running buffer. The mechanism of lipid-based nanoparticles affecting bacterial adhesion and aggregation was discussed and supported by zeta potential experiments. Under the optimal conditions, the three species of bacteria were identified with patterned peaks. This proposed MCE method using lipid-based nanoparticles as running buffer additives was also used to analyse a real yogurt sample, and valuable bacterial information was obtained by the electropherograms.
Keywords :
Microchip electrophoresis , Bacteria analysis , Lipid-based nanoparticles
Journal title :
Journal of Chromatography A
Serial Year :
2011
Journal title :
Journal of Chromatography A
Record number :
1514663
Link To Document :
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