Title of article :
Analysis of Salvinorin A in plants, water, and urine using solid-phase microextraction-comprehensive two-dimensional gas chromatography–time of flight mass spectrometry
Author/Authors :
Barnes، نويسنده , , Brian B. and Snow، نويسنده , , Nicholas H.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
Salvinorin A, a psychoactive hallucinogen, and related compounds, were analyzed in plants, water, and urine using liquid–liquid extraction (LLE), solid-phase microextraction (SPME) and comprehensive two-dimensional gas chromatography–time of flight mass spectrometry (GC × GC–ToFMS). A semi-qualitative study of the extraction of Salvinorin A and analogs from Salvia divinorum plants by LLE showed ppb levels of Salvinorin A and several analogs in the leaves and stems of S. divinorum plants, much lower than expected. Quantitative analysis of Salvinorin A spiked into water and urine showed much better figures of merit for SPME than LLE, with limit of detection of about 5 ng/mL, linear range from 8 to 500 ng/mL and precision about ±10% for the SPME-based analyses using external standard quantitation. GC × GC–ToFMS was especially effective in separating the peaks of interest from matrix and chromatographic interferences.
Keywords :
Salvinorin A , Salvia divinorum , × , SPME , GC , GC , GC , × , GC–TOFMS , Sage
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A