Author/Authors :
Macmaster، نويسنده , , Angus P. and Owen، نويسنده , , Neil and Brussaux، نويسنده , , Sylvain and Brevard، نويسنده , , Hugues and Hiserodt، نويسنده , , Richard and Leijs، نويسنده , , Hans and Bast، نويسنده , , Nikola and Weber، نويسنده , , Berthold and Loesing، نويسنده , , Gerd and Sherlock، نويسنده , , Alan and Schippa، نويسنده , , Christine and Vey، نويسنده , , Matthias and Frérot، نويسنده , , Eric and Tiss، نويسنده ,
Abstract :
The performance of HPLC–UV as a means of quantifying selected furocoumarins in essential oils has been evaluated, based on a ring test validation approach. Accuracy profiles were generated, to determine bias and statistical confidence associated with determination at different concentrations, along with lower limits of quantification (LOQ). From these findings, it can be concluded that the method described may only be used in simple cases (essential oils), to measure individual furocoumarin compounds at concentrations greater than 10 mg/l; the non compound-specific nature of detection by absorption in the UV range is unable to overcome the effect of interferences arising from chromatographic coelutions, such as those encountered in the analysis of complex commercial fragrance mixtures. The use of an algorithmically calculated ‘spectral similarity’ function, with reference to authentic standards, may be used to improve reliability in assignment and quantification.
Keywords :
HPLC–UV , accuracy , Quantification , Limit of quantification , Furocoumarin , Validation
