Author/Authors :
Leonard، نويسنده , , Stefanie and Capote، نويسنده , , Raul and Germonprez، نويسنده , , Nils and Van Puyvelde، نويسنده , , Luc and De Kimpe، نويسنده , , Norbert and Vermeersch، نويسنده , , Hans and Rosier، نويسنده , , Hendrik-Jan and Maes، نويسنده , , Louis and Roets، نويسنده , , Eugène and Hoogmartens، نويسنده , , Jos، نويسنده ,
Abstract :
A liquid chromatographic method was developed for the separation of six related triterpenoid saponins in Maesa balansae extracts with different purity, active against leishmaniasis. As stationary phase a Hypersil BDS C18 column (3 μm), 100×4.6 mm was used. The mobile phase was a mixture of methanol, acetonitrile, 5% (m/v) ammonium acetate, pH 6.5 and water. A linear gradient was developed for the analysis of crude extracts. An isocratic method was developed to analyze purified samples that mainly contained saponins 3 and 4, the most active saponins. The isocratic LC method was optimized and the robustness was evaluated with an experimental design. The method showed good selectivity, repeatability, linearity and sensitivity.
