Author/Authors :
Yang، نويسنده , , Changming and Shimelis، نويسنده , , Olga and Zhou، نويسنده , , Xiaojuan and Li، نويسنده , , Guoding and Bayle، نويسنده , , Christophe and Nertz، نويسنده , , Michel and Lee، نويسنده , , Hyeran and Strekowski، نويسنده , , Lucjan and Patonay، نويسنده , , Gabor and Couderc، نويسنده , , Francois and Giese، نويسنده , , Roger W، نويسنده ,
Abstract :
We are interested in the detection of DNA adducts and other trace analytes by labeling them with a fluorescent tag followed by use of capillary electrophoresis with laser-induced fluorescence detection (CE–LIF) for high resolution and sensitivity. Towards this goal, here we report the following: (1) synthesis and handling properties of a near-IR, carboxyl-substituted heptamethine cyanine dye; (2) modification of an existing ball lens LIF detector to provide near-LIF detection with excitation at 785 nm for CE; and (3) corresponding handling and detection of as little as 0.8 amol of the dye by enrich-injection of 4.7 μl of 1·10−13 mol/l dye in methanol from an 8-μl volume into a corresponding CE–LIF system. The electrolyte for the separation was methanol–40 mmol/l aqueous sodium borate (98:2, v/v). This finding encourages further exploration of the dye by functionalization of its carboxyl group for chemical labeling purposes.
