Author/Authors :
Kaiser، نويسنده , , Thorsten and Hermann، نويسنده , , Alena and Kielstein، نويسنده , , Jan T. and Wittke، نويسنده , , Stefan and Bartel، نويسنده , , Sebastian and Krebs، نويسنده , , Ronald and Hausadel، نويسنده , , Frank and Hillmann، نويسنده , , Meike and Golovko، نويسنده , , Igor and Koester، نويسنده , , Peer and Haller، نويسنده , , Hermann and Weissinger، نويسنده , , Eva M. and Fliser، نويسنده , , Danilo and Mischak، نويسنده , , Harald، نويسنده ,
Abstract :
Combination of capillary electrophoresis with mass spectrometry (CE–MS) allows generation of polypeptide patterns of body fluids. In a single CE–MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (Mr>10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected. Comparison to the normal urine pattern yielded a surprisingly low consensus: a number of polypeptides present in urine were missing. We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers.
