Characterization of three Agave species by gas chromatography and solid-phase microextraction–gas chromatography–mass spectrometry

Steam distillation (SD) extraction–solid-phase microextraction coupled to GC–MS was developed for the determination of terpenes and Bligh–Dyer extraction–derivatization coupled with GC for the determination of fatty acids such as ethyl esters were used. It was found that the three different Agave species have the same profile of fatty acids; the quantity of these compounds is different in each Agave variety. On the other hand, different terpenes were identified in the three Agave plants studied: nine in A. salmiana, eight in A. angustifolia and 32 in A. tequilana Weber var. azul.