Author/Authors :
Ventura، نويسنده , , Manuel and Farrell، نويسنده , , William and Aurigemma، نويسنده , , Christine and Tivel، نويسنده , , Kathleen and Greig، نويسنده , , Michael and Wheatley، نويسنده , , Jeffrey and Yanovsky، نويسنده , , Alex and Milgram، نويسنده , , Kenneth Eric and Dalesandro، نويسنده , , David and DeGuzman، نويسنده , , Raylyn and Tran، نويسنده , , Phuong and Nguyen، نويسنده , , Leslie and Chung، نويسنده , , Loanne and Gron، نويسنده , , Ole and Koch، نويسنده , , Charles A.، نويسنده ,
Abstract :
A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded.
Keywords :
Parallel synthesis , instrumentation , Preparative chromatography , Supercritical fluid chromatography
