Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography

Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata was conducted using high-speed counter-current chromatograph (HSCCC) with a solvent system composed of n-hexane–ethyl acetate–methanol–water (1:6:1.5:7.5, v/v). In a single operation, 28 mg of 5,7-dihydroxy-3′,4′-trihydroxyflavone-3-O-6′′-rhamnose and 18 mg of 5,7-dihydroxy-3′,4′-dihydroxyflavone-3-O-6′′-rhamnose was obtained from 150 mg of the extract. The chemical structure of the two compounds was elucidated by electrospray ionization (EIS) MS and NMR.