Title of article :
Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device
Author/Authors :
Baldock، نويسنده , , S.J and Fielden، نويسنده , , P.R and Goddard، نويسنده , , N.J and Kretschmer، نويسنده , , H.R and Prest، نويسنده , , J.E and Treves Brown، نويسنده , , B.J، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
8
From page :
181
To page :
188
Abstract :
A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.
Keywords :
Isotachophoresis , Miniaturization , Injection methods , instrumentation , Chip technology
Journal title :
Journal of Chromatography A
Serial Year :
2004
Journal title :
Journal of Chromatography A
Record number :
1520596
Link To Document :
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