Author/Authors :
Ito، نويسنده , , Yoichiro and Qi، نويسنده , , Lin and Powell، نويسنده , , Jimmie and Sharpnack، نويسنده , , Frank and Metger، نويسنده , , Howard and Yost، نويسنده , , James and Cao، نويسنده , , Xue-Li and Dong، نويسنده , , Yin-Mao and Huo، نويسنده , , Liangsheng and Zhu، نويسنده , , Xiao-Ping and Li، نويسنده , , Ting، نويسنده ,
Abstract :
A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm.
Keywords :
Barricaded spiral disk , Coil planet centrifuge , Assembly , Purification of biopolymers , Mixer-settler counter-current chromatography
