Title of article
Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography
Author/Authors
Myrdal، نويسنده , , Paul B. and Angersbach، نويسنده , , B. Steven and Karlage، نويسنده , , Kelly and Kuehl، نويسنده , , Philip J.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
5
From page
315
To page
319
Abstract
An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.
Keywords
Arachidonic acid metabolism , lipoxygenase , HETEs , Chiral separation , Chiralcel OR-RH
Journal title
Journal of Chromatography A
Serial Year
2006
Journal title
Journal of Chromatography A
Record number
1521917
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