Stationary phase optimized selectivity liquid chromatography: Basic possibilities of serially connected columns using the “PRISMA” principle

A new procedure (stationary phase optimized selectivity liquid chromatography: SOS–LC) is described for the optimization of the HPLC stationary phase, using serially connected columns and the principle of the “PRISMA” model. The retention factors (k) of the analytes were determined on three different stationary phases. By use of these data the k values were predicted applying theoretically combined stationary phases. These predictions resulted in numerous intermediate theoretical separations from among which only the optimal one was assembled and tested. The overall selectivity of this separation was better than that of any individual base stationary phase. SOS–LC is independent of the mechanism and the scale of separation.