Use of gas chromatography–mass spectrometry for identification of a new disaccharide in honey

Gas chromatography–mass spectrometry has been used to separate and identify disaccharides of edible honey. According to the characteristic fragmentation behaviour of disaccharide TMS-oximes, fructofuranosyl-(2-1)-fructose (inulobiose) has been structurally characterized. Identification was carried out on the basis of retention time on two columns of different polarity and mass spectrometric analysis. Inulobiose was found in honeys of different origins for the first time, varying within 0.93 and 6.14 mg/g of honey. Its occurrence in honeys is discussed.