Ion-pair chromatographic separation of glycoprotein derived oligosaccharides as their 8-aminopyrene-1,3,6-trisulfonic acid derivatives

A reversed-phase ion-pair gradient liquid chromatographic method has been developed for separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeled oligosaccharides derived from some glycoproteins. This separation mode has high capability for structural recognition of oligosaccharide isomers, which are usually difficult to separate using commonly used partition chromatography. In addition, some glycoprotein-derived complex-type oligosaccharides that have linkage isomers in one lactosamine arm and neuraminic acids existing in fetuin, as well as differences in the numbers of lactosamine branches, are separable using this mode. The ion-pair mode is promising for simple analyses of glycoprotein-derived oligosaccharides that are labeled with APTS.