Title of article
A high performance liquid chromatography assay for monitoring proprotein convertase activity
Author/Authors
Hall، نويسنده , , Troii and Fok، نويسنده , , Kam F. and Liu، نويسنده , , Min M. and Zobel، نويسنده , , James F. and Marino، نويسنده , , Margaret H. and Malfait، نويسنده , , Anne-Marie and Tortorella، نويسنده , , Micky D. and Tomasselli، نويسنده , , Alfredo G.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
9
From page
46
To page
54
Abstract
A rapid HPLC assay was developed for monitoring the activity of the two proprotein convertases, PACE-4 and furin. Six novel peptide substrates were synthesized containing the minimal PC recognition sequence (Arg-X-X-Arg), as well as tryptophan residue(s) for easy detection. Four of the peptides were cleaved by both PCs and their kinetic parameters determined. Two peptides were not cleaved but were shown to be good negative controls although not inhibitors of either PC. In addition, inhibition curves were plotted and IC50 values calculated for PACE-4 and furin in the presence of two polyarginine peptides, hexa and deca-D-arginine.
Keywords
Proprotein convertases , PACE-4 , Furin , HPLC
Journal title
Journal of Chromatography A
Serial Year
2007
Journal title
Journal of Chromatography A
Record number
1523187
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