Title of article :
State of the art of shear driven chromatography: Advantages and limitations
Author/Authors :
Fekete، نويسنده , , Veronika and Clicq، نويسنده , , David and De Malsche، نويسنده , , Wim and Gardeniers، نويسنده , , Han and Desmet، نويسنده , , Gert، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
10
From page :
2
To page :
11
Abstract :
The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.
Keywords :
Shear-driven chromatography , CCD camera , PMT , Separation of coumarin dyes , Plate height , Coating
Journal title :
Journal of Chromatography A
Serial Year :
2007
Journal title :
Journal of Chromatography A
Record number :
1523219
Link To Document :
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