Title of article :
Liquid chromatography coupled with inductively coupled plasma mass spectrometry in the pharmaceutical industry: selected examples
Author/Authors :
Marshall، نويسنده , , Peter S. and Leavens، نويسنده , , Bill and Heudi، نويسنده , , Olivier and Ramirez-Molina، نويسنده , , Cesar، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
10
From page :
3
To page :
12
Abstract :
Both LC and capillary LC (CapLC) have been successfully interfaced with inductively coupled plasma mass spectrometry (ICP-MS). Gradients of acetonitrile and aqueous based solvents have been employed to separate several compounds of pharmaceutical interest. This paper will describe four application areas in the pharmaceutical industry, and examples will be shown where CapLC, LC and gel electrophoresis via laser ablation have been coupled with ICP-MS. The four areas highlighted in this paper are: (1) the use of derivatisation reactions to “make the invisible visible”. Methods involving derivatisations with copper and iron will be described that can be used for the analysis of amines and carboxylic acids by ICP-MS. (2) The profiling of metal ion content (in particular bromine) in biological samples such as human plasma, this study will focus on the metabolism of bromine-labelled peptides (e.g. substance P). (3) The analysis of materials derived from single, solid-phase beads used in combinatorial chemistry, and (4) also discussed will be our findings from investigations into the use of laser ablation ICP-MS on the determination of protein phosphorylation on electrophoresis gel blots.
Keywords :
Inductively coupled plasma mass spectrometry (ICP-MS) , Derivatisation , substance P
Journal title :
Journal of Chromatography A
Serial Year :
2004
Journal title :
Journal of Chromatography A
Record number :
1523562
Link To Document :
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