Determination of adsorption isotherms of proteins by H-root method: Comparison between open micro-channels and conventional packed columns

This communication compares the accuracy of a micro open parallel plate system (μOPPS) with a conventional packed column for predicting isotherm data by using the H-root method (HRM). HRM is restricted to compounds obeying the Langmuir isotherm model. The performance of the two chromatographic systems was simulated by using comprehensive mathematical models. Operating conditions were varied and their effects on the accuracy of predictions was evaluated. Better accuracy in the isotherm predictions was obtained with the packed column due to its higher efficiency. However, good predictions can be obtained with the μOPPS with the advantage of significantly lower sample consumption.