Title of article :
Rapid separation of antimicrobial metabolites by microchip electrophoresis with UV linear imaging detection
Author/Authors :
Guihen، نويسنده , , Elizabeth and Glennon، نويسنده , , Jeremy D.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
6
From page :
223
To page :
228
Abstract :
This research examines microchip electrophoresis with linear imaging UV detection for the analysis of antimicrobial metabolites, monoacetylphloroglucinol (MAPG) and 2,4-diacetylphloroglucinol (2,4-DAPG) from Pseudomonas fluorescens F113. Initial results show the separation of MAPG, 2,4-DAPG and resorcinol in less than 20 s. This was achieved using a quartz microchip with a separation channel length of 25 mm. In order to quantitate the amount of MAPG and 2,4-DAPG in a microbial cultured supernatant sample, on-chip sample introduction in a methanol/buffer matrix was investigated. Sample introduction/injection parameters were optimized to improve sensitivity and thus decrease the limit of detection (LOD). The amount of antimicrobial metabolites present was quantitated with a separation time of 15 s. A previously developed capillary electrophoretic method was compared to the microchip method in relation to speed, efficiency, precision, linear range and limit of detection. This investigation shows the fastest separation so far of these antimicrobial metabolites with high efficiency.
Keywords :
2 , Monoacetylphloroglucinol (MAPG) , 4-Diacetylphloroglucinol (2 , 4-DAPG) , Microchip electrophoresis (MCE) , Pseudomonas fluorescens F113 , Antimicrobial metabolites
Journal title :
Journal of Chromatography A
Serial Year :
2005
Journal title :
Journal of Chromatography A
Record number :
1523939
Link To Document :
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