Chromatographic, capillary electrophoretic and capillary electrochromatographic techniques in the analysis of flavonoids

An overview is presented of chromatographic methods currently in use to determine flavonoids, including free aglycones, their corresponding glycosides, one by one, and, in the presence of each other. As a basis of selection, the following approaches can be distinguished: critical evaluation of the preliminary steps (extraction/isolation and hydrolysis) as well as the separation, identification and quantitation of constituents both on the basic research level and/or subsequently to various work up procedures. Chromatographic techniques were discussed after extraction/isolation of various flavonoids from several natural matrices. Papers were classified and compared from analytical point of view, primarily on the chromatographic, secondly on the detection techniques applied.