Solvent versatility of immobilized 3,5-dimethylphenylcarbamate of amylose in enantiomeric separations by HPLC

CHIRALPAK® IA is a new chiral stationary phase containing amylose 3,5-dimethylphenylcarbamate immobilized onto silica gel. It is compatible with the whole range of organic solvents. Its solvent versatility has been thoroughly investigated. The option to use a wide range of solvents, especially the “non-standards” ones, in the mobile phase enables the enhancement of chiral separation methods in terms of enantioselectivity, resolution, analysis time, sample injection and sample solubility. Parameters such as the mobile phase type, the nature of modifier and eluting strength of various solvents are examined and discussed. A guideline for method development and optimization on CHIRALPAK® IA is also proposed.