Title of article :
Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC
Author/Authors :
Su، نويسنده , , Xingye and Kong، نويسنده , , Liang and Li، نويسنده , , Xin and Chen، نويسنده , , Xueguo and Guo، نويسنده , , Ming and Zou، نويسنده , , Hanfa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Abstract :
Biofingerprinting chromatogram analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional Chinese medicines (TCMs) before and after the interaction with biological systems (DNA, protein, cell, etc.), was proposed for screening and analysis of the multiple bioactive compounds in TCMs. A method of microdialysis sampling combined with high performance liquid chromatography (HPLC) was applied to the study of DNA-binding property for the extracts of TCMs. Seven compounds were found to bind to calf thymus DNA (ct-DNA) from the TCMs of Coptis chinensis Franch (Coptis), but only three ones from Phellodendron amurense Rupr. (Phellodendron) and none from Sophora flavescens Ait. (Sophora) to bind to ct-DNA, respectively. Three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (K) to ct-DNA were determined by microdialysis/HPLC. Competitive binding behaviors of them to ct-DNA were also investigated.
Keywords :
Bioactive compounds , Biofingerprinting analysis , Small molecule-DNA binding , Microdialysis/HPLC , Traditional Chinese medicines
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A